Table 2 Primers used for the detection of Campylobacter spp., Salmonella spp. and Listeria monocytogenes, as well as the detection and characterisation of Vibrio cholerae
From: Diarrheagenic pathogens in adults attending a hospital in Singapore
Parameter | Target gene | Forward primer (5ā²-3ā²) | Reverse primers (5ā²-3ā²) | Concentration (Ī¼M) | Size (bp) | References |
|---|---|---|---|---|---|---|
Aeromonas spp. | 16Ā s rDNA | GGGAGTGCCTTCGGGAATCAGA | TCACCGCAACATTCTGATTTG | 0.5 | 356 | [14] |
Campylobacter spp. | 16Ā s rDNA | GGTGTAGGATGAGACTATATA | TTCCATCTGCCTCTCCCY | 0.5 | 439 | [54] |
Salmonella spp. | invA | ACAGTGCTCGTTTACGACCTGAAT | AGACGACTGGTACTGATCGATAAT | 0.5 | 244 | [55] |
Listeria monocytogenes | InlA | ACGAGTAACGGGACAAATGC | CCCGACAGTGGTGCTAGATT | 0.5 | 800 | [56] |
V. cholerae | toxR | GAAGCTGCTCATGACATC | AAGATCAGGGTGGTTATTC | 0.01 | 275 | [57] |
V. cholerae serogroup O1 | Wbe (O1) | GTTTCACTGAACAGATGGG | GGTCATCTGTAAGTACAAC | 0.05 | 192 | [15] |
V. cholerae biotype (El Tor) | tcpA | CACGATAAGAAAACCGGTCAAGAG | CGAAAGCACCTTCTTTCACGTTG | 0.05 | 451 | [58] |
V. cholerae virulence genes | ctxA | ACAGAGTGAGTACTTTGACC | ATACCATCCATATATTTGGGAG | 0.05 | 308 | [15] |
| Ā | ctxB | ATGAGGCGTTTTATTATTCCATACAC | TACCAGGTAGTCAACATATAGATTCA | 0.05 | 128 | [59] |