Fig. 1

Characterization of the viral-enriched fraction of DV-infected HepG2 supernatant or plasma obtained from patients with dengue. a After infection with DV at a MOI of 1 or 10, HepG2 cell culture supernatants were purified by ultracentrifugation (Step 1) followed by the use of Viraffinity™ (Step 1 + 2). b Silver-staining assessment of the protein complexity of DV-infected HepG2 cell culture supernatant before and after purification; not diluted (lane “not purified”), 1/10 diluted (lane “not purified 1/10”) DV-infected cell supernatant before purification or the same sample as shown in lane 1 after purification (lane “Step 1 + 2”) were separated by denaturing polyacrylamide gel electrophoresis and silver-stained. c Electron microscopy of DV-infected HepG2 culture supernatant after ultracentrifugation. Grids were negatively stained using uranyl acetate. Bars: 50 nm, 200 nm. d Representative Western blotting analysis of plasma pools obtained from patients with dengue after purification by ultracentrifugation and Viraffinity™ using an anti-E monoclonal antibody. MW: molecular mass