Assessing the impact of pneumococcal conjugate vaccines on invasive pneumococcal disease using polymerase chain reaction-based surveillance: an experience from South Africa

Table 1 Characteristics of S. pneumoniae-positive cases (N = 36) hospitalized at Chris Hani Baragwanath Academic Hospital for which a serotype/serogroup could be identified by the Quellung reaction and/or the molecular serotyping assay, Soweto, South Africa, 2009-2012^a

Age group (in years)	HIV serostatus	lytA Ct-value	Serotype/serogroup
Age group (in years)	HIV serostatus	lytA Ct-value	Quellung reaction	Molecular serotyping assay
lytA Ct-value ≤30
25–44	Pos	25	19 F	19B/19 F
45–64	Pos	26	1	1
<2	Pos	27	10A^c	Neg42
25–44	Pos	27	19 F	19B/F
25–44	Unknown	27	Not available^b	18A/B/C
25–44	Pos	27	19A	19A
25–44	Pos	28	19A	19A
25–44	Pos	28	19 F	19B/F
25–44	Pos	29	19A	19A
25–44	Pos	29	19A	19A
25–44	Pos	30	19A	19A
lytA Ct-value 31–34
25–44	Pos	31	3	3
45–64	Unknown	31	19A	19A
25–44	Pos	31	1	1
25–44	Pos	32	12 F	12A/B/F
25–44	Neg	32	19A	19A
45–64	Pos	33	4	4
25–44	Pos	33	Not available^b	19A
25–44	Neg	34	1	1
25–44	Pos	34	Not available^b	1
25–44	Pos	34	1^d	Neg42
45–64	Pos	34	19A	19A
lytA Ct-value ≥35
5–24	Pos	35	9 V	9A/L/N/V
<2	Pos	35	23 F	23 F
5–24	Pos	35	19A^d	Neg42
25–44	Pos	35	1^d	Neg42
<2	Neg	36	6A	6A/B
<2	Unknown	36	6B	6A/B
5–24	Pos	36	18C^d	Neg42
25–44	Pos	37	19A^d	Neg42
2–4	Neg	37	14^d	Neg42
25–44	Pos	37	Not available^b	1
25–44	Pos	38	1^d	Neg42
25–44	Pos	38	23A^c	Neg42
2–4	Pos	39	6A^d	Neg42
<2	Neg	39	19A^e	18A/B/C^e

Abbreviations: HIV: human immunodeficiency virus; Ct-value: cycle threshold value; Neg42: samples that tested negative for the 42 serotypes detected by the serotyping assay
^a Discrepant or missing serotype/serogroup results are in bolt font
^b Isolate not available for serotyping using the Quellung reaction
^c Serotype not included in the molecular serotyping assay
^d Serotype included in the molecular serotyping assay

ISSN: 1471-2334