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Table 2 Accurate reproducibility of the Kalon HSV-2 IgG ELISA (N = 183; HSV-2 prevalence = 72 %) a

From: Precision of the Kalon Herpes Simplex Virus Type 2 IgG ELISA: an international inter-laboratory assessment

Kalon conditions Sensitivity, % (95 % CI) Specificity, % (95 % CI) AUC (95 % CI)
IND as NEG b
 Technologist-Jr. 90.1 (83.6–94.6) 100.0 (92.7–100.0) 0.95 (0.93–0.98)
 Technologist-Sr. e 92.4 (86.4–96.3) 95.9 (86.0–99.5) 0.94 (0.91–0.98)
 Technologist-Sr. f 94.7 (89.3–97.8) 98.0 (89.1–99.9) 0.96 (0.94–0.99)
 Technologist-SA 88.5 (81.8–93.4) 100.0 (92.7–100.0) 0.94 (0.92–0.97)
 Technologist-ZM 93.1 (87.4–96.8) 98.0 (89.1–99.9) 0.96 (0.93–0.98)
IND as POS c
 Technologist-Jr. 93.1 (87.4–96.8) 98.0 (89.1–99.9) 0.96 (0.93–0.99)
 Technologist-Sr. e 94.7 (89.3–97.8) 95.9 (86.0–99.5) 0.95 (0.91–0.99)
 Technologist-Sr. f 96.2 (91.3–98.7) 98.0 (89.1–99.9) 0.97 (0.95–1.00)
 Technologist-SA 91.6 (85.5–95.7) 95.9 (86.0–99.5) 0.94 (0.90–0.97)
 Technologist-ZM 94.7 (89.3–97.8) 98.0 (89.1–99.9) 0.96 (0.94–0.99)
IND excluded d
 Technologist-Jr. 92.9 (87.0–96.7) 100.0 (92.6–100.0) 0.97 (0.94–0.99)
 Technologist-Sr. e 94.5 (89.1–97.8) 95.9 (86.0–99.5) 0.95 (0.92–0.99)
 Technologist-Sr. f 96.1 (91.2–98.7) 98.0 (89.1–99.1) 0.97 (0.94–1.00)
 Technologist-SA 91.3 (85.0–95.6) 100.0 (92.5–100.0) 0.96 (0.93–0.98)
 Technologist-ZM 94.6 (89.1–97.8) 98.0 (89.1–99.9) 0.96 (0.94–0.99)
  1. Abbreviations: POS positive, NEG negative, IND indeterminate, CI confidence interval, AUC area under the receiver operating characteristic curve
  2. aGold standard: UW-WB; indeterminate results by UW-WB were excluded in the analysis (n = 3)
  3. bIndeterminate results by Kalon were considered as negative in the analysis
  4. cIndeterminate results by Kalon were considered as positive in the analysis
  5. dIndeterminate results by Kalon were excluded in the analysis
  6. eKalon was performed at the HPTN Laboratory Center (USA site)
  7. fKalon was performed in the South African laboratory
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