Table 3 Diagnostic accuracy results of the NS1 Ag-based tests on the viral serotype, serological status and clinical severity

Overall

 No. of study

11

5

14

7

 Sensitivity % (95 % CI)

66.2 (64.1–68.2)

54 (50.1–57.9)

63.3 (61.5–65.1)

72.9 (70.1–75.5)

 Specificity % (95 % CI)

97.3 (96.1–98.2)

97.2 (95.2–98.6)

97.3 (96.3–98.1)

99.1 (98.2–99.6)

 Heterogeneity % (I²)

84.1

79.8

82.4

40.5

DENV serotypes

 DENV1

  No. of study

7

2

8

3

  Sensitivity % (95 % CI)

83.7 (79.7–87.1)

73.7 (65.5–80.9)

81.2 (77.7–84.4)

75.9 (70.1–81)

  Heterogeneity % (I²)

82.5

89.6

84

57.8

 DENV2

  No. of study

7

2

8

4

  Sensitivity % (95 % CI)

56.9 (51.9–61.8)

71.8 (60.5–81.4)

59.3 (54.8–63.7)

69 (63–74.5)

  Heterogeneity % (I²)

88.6

0

86.4

70.4

 DENV3

  No. of study

6

2

7

4

  Sensitivity % (95 % CI)

69.9 (64.8–74.6)

63.3 (48.3–76.6)

69.1 (64.3–73.5)

81.9 (75.5–87.2)

  Heterogeneity % (I²)

82.3

15.3

76.9

75.5

 DENV4

  No. of study

4

0

4

0

  Sensitivity % (95 % CI)

42.9(32.1–54.1)

42.9 (32.1–54.1)

  Heterogeneity % (I²)

57.1

57.1

Serological status

 Primary

  No. of study

7

2

8

2

  Sensitivity % (95 % CI)

95.1 (92.6–96.9)

72.6 (65.6–78.9)

88.4 (85.7–90.8)

87.3 (83.6–90.5)

  Heterogeneity % (I²)

56.8

29.2

89.1

94.6

 Secondary

  No. of study

7

3

9

2

  Sensitivity % (95 % CI)

63 (59.5–66.4)

50.8 (43.5–58)

60.6 (57.4–63.6)

64 (53.2–73.9)

  Heterogeneity % (I²)

95.8

0

94.1

88.1

Clinical severity

 Dengue Fever

  No. of study

4

1

5

1

  Sensitivity % (95 % CI)

72.1 (67.6–76.3)

51 (37–64)

69.5 (65.2–73.6)

89 (67–99)

  Heterogeneity % (I²)

73.9

81.7

 Dengue Hemorrhagic Fever

  No. of study

4

1

5

1

  Sensitivity % (95 % CI)

58.7 (52.7–64.5)

58.7 (49–67)

58.6 (53.6–63.4)

61 (43–77)

  Heterogeneity % (I²)

82.9

77.2