Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

Table 1 Primers and PNA probes used for identification of Mycobacterium abscessus group in the present study

Primer/Probe	Sequence (5′ → 3′)	T_m (°C)^a	Potential target organisms
Primer
Abs/Mas-F	CCGAGACGCTGCTGAAGAG	66.2	M. abscessus, M. massiliense
Abs/Mas-R	GACGTCCTCGGCGATGAT	65.5	M. abscessus, M. massiliense
PNA Probe
Mas/Abs	Dabcyl-CCTCGTTACCAACCT-O-K-FAM^b	66.8	M. massiliense, M. abscessus
Mas-T2	Dabcyl-GGAGATTCCGGCC-O-K-Hex	69.5	M. massiliense Type 2/Type 1
Mas-T2-1	Dabcyl-CTGAATTACCTTCTC-O-K-Texas Red	62.0	M. massiliense Type 2-1/Type 2-2

^aPrimer T_m was calculated by using Oligo software V 6.50 and probe T_m was calculated by http://pnabio.com/support/PNA_Tool.htm; T_m, melting temperature
^bK, lysine; O, O linker

ISSN: 1471-2334