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Table 1 Primers and PNA probes used for identification of Mycobacterium abscessus group in the present study

From: Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

Primer/Probe Sequence (5′ → 3′) T m (°C)a Potential target organisms
Primer    
 Abs/Mas-F CCGAGACGCTGCTGAAGAG 66.2 M. abscessus, M. massiliense
 Abs/Mas-R GACGTCCTCGGCGATGAT 65.5 M. abscessus, M. massiliense
PNA Probe    
 Mas/Abs Dabcyl-CCTCGTTACCAACCT-O-K-FAMb 66.8 M. massiliense, M. abscessus
 Mas-T2 Dabcyl-GGAGATTCCGGCC-O-K-Hex 69.5 M. massiliense Type 2/Type 1
 Mas-T2-1 Dabcyl-CTGAATTACCTTCTC-O-K-Texas Red 62.0 M. massiliense Type 2-1/Type 2-2
  1. aPrimer T m was calculated by using Oligo software V 6.50 and probe T m was calculated by http://pnabio.com/support/PNA_Tool.htm; T m , melting temperature
  2. bK, lysine; O, O linker