Fig. 3

Antigen-specific responses among the broader population. Sera from multiple groups were analyzed for antibodies against a. LID-1 (IgG); b. NDO-LID (IgG and IgM); c. NDO-HSA (IgM) and d. PGL-I (IgM). Each point represents the EI of an individual serum sample, with the total number of participants in each group noted below. The mean EI is represented by the horizontal line. The traced horizontal line is threshold for determining a positive result (EI = 1.1). a: IgG against LID-1: Patients versus HHCs versus Gpop, difference not significant (H = 0.57; p = 0.753). b: IgG and IgM against NDOLID: Patients versus HHCs versus Gpop (H = 31.37). Mann–Whitney U test with Bonferroni correction: Patients versus HHCs (p < 0.0001); Patients versus GPop (p < 0.0001); HHCs versus GPop * mean EI of the GPop group was greater than the mean EI of the HHCs group (p < 0.0001). c: IgM against NDOHSA: Patients versus HHCs versus GPop (H = 50.66). Mann–Whitney U test with Bonferroni correction: Patients versus HHCs (p < 0.0001); Patients versus GPop (p = 0.013); HHCs versus GPop * mean EI of the EC group was greater than the mean EI of the HHCs group (p < 0.0001). d: IgM against PGL-1: Patients versus HHCs (U = 1769; p < 0.0001)