Table 1 Analytical sensitivity and specificity showed by the different ELISA and IIF assays used by the participant laboratories
From: International external quality control assessment for the serological diagnosis of dengue infections
IgM sensitvity | IgM specificity | IgG sensitivity | IgG specificity | |
|---|---|---|---|---|
Overall* | 66.7% (95% CI 62.3%-70.8%) | 95.1% (95% CI 92.2%-97.2%) | 84.4% (95% CI 80.6%-87.7%) | 83% (95% CI 78%-87.1%) |
ELISA assays | 70.4% (95% CI 65.5%-75%) | 97.2% (95% CI 94.4%-98.9%) | 83.2% (95% CI 78.5%-87.3%) | 83.8% (95% CI 78%-88.7%) |
ELISA commercial | 75.5% (95% CI 70.3%-80.2%) | 97% (95% CI 93.7%-98.9%) | 82.4% (95% CI 77.3%-86.8%) | 84.5% (95% CI 78.2-89.5%) |
Capture assays | 82.7% (95% CI 77%-87.4%) | 96% (95% CI 91.5%-98.6%) | 59.3% (95% CI 45%-72.4%) | 97.2% (95% CI 85.4%-99.5%) |
Indirect assays | 55.6% (95% CI 44%-66.6%) | 100% (95% CI 93.3%-100%) | 88.9% (95% CI 83.9%-92.9%) | 82.6% (95% CI 75.2%-88.5%) |
ELISA in house | 48.6% (95% CI 35.6%-60.7%) | 97.9% (95% CI 88.9%-99.6%) | 88.9% (95% CI 73.9%-96.8%) | 79.2% (95% CI 57.8%-92.8%) |
IIF assays | 56.6% (95% CI 46.2%-66.5%) | 86.4% (95% CI 75.7%-93.6%) | 88.9% (95% CI 81.7%-93.9%) | 79.5% (95% CI 68.8%-87.8%) |
IIF commercial | 59.7% (95% CI 47.5%-71.1%) | 97.9% (95% CI 88.9%-99.6%) | 88.9% (95% CI 79.9%-94.8%) | 77.7% (95% CI 64.4%-87.9%) |
IIF in house | 48.1% (95% CI 28.7%-68%) | 55.5% (95% CI 30.8%-78.4%) | 88.9% (95% CI 73.9%-96.8%) | 83.3% (95% CI 62.6%-95.2%) |