Table 1 DPO primers used for multiplex DPO PCR
Virus | Target gene | Primer sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|---|
H1N1 pdm09 | HA | F:TAGTGCTGACCAACAAAGTCTCTAIIIIIATGCAG | 231 |
R:GCATTTCTTTCCATTGCGAATGCIIIIITCGGTAC | |||
sH1N1 | HA | F:TGCGAATYACTGATTTCCAAGGAIIIIIGGTCCTA | 193 |
R: CTCCGGTTACRGTGTGGTGGGGIIIIIAGCTCTCT | |||
H3N2 | HA | F:ACGCTGTGCCTTGGGCACCATGCAIIIIIAAACGG | 537 |
R:GTCMTTGTCCGTACCCGGGTGGTGIIIIICCCAAA | |||
H5N1 | HA | F:GAGAGATTGTAGTGTAGCTGGATIIIIICTCGGAA | 328 |
R:CTTTATTGTTGGGTATGTRCTGTTIIIIITGATAAGCC | |||
FluB | PB1 | F:TTGGCTATGACTGAAAGAATAACCAIIIIIAGCCCA | 401 |
R:GCATTAACAAATAGAGCAAAATCATIIIIIGATTGC |