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Table 1 Oligonucleotide primers, probes and internal control sequences

From: Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples

Assay

Name

Sequence

qPCR

Sp-ply-531 F1

AGCGATAGCTTTCTCCAAGTGG

 

Sp-ply-583R1

CTTAGCCAACAAATCGTTTACCG

 

Sp-lytA-306 F1

ACGCAATCTAGCAGATGAAGC

 

Sp-lytA-386R1

TGTTTGGTTGGTTATTCGTGC

 

Sp-lytA-CDCF2

ACGCAATCTAGCAGATGAAGCA

 

Sp-lytA-CDCR2

TCGTGCGTTTTAATTCCAGCT

 

Sp-ply-556probe1

FAM-ACCCCAGCAATTCAAGTGTTCGCG-BHQ1

 

Sp-lytA330-probe1

Cy5-TTTGCCGAAAACGCTTGATACAGGG-BHQ3

 

Sp-lytA-CDCprobe2

FAM-GCCGAAAACGCTTGATACAGGGAG-BHQ1

 

Sp-lytA-probe2

FAM-TTTGCCGAAAACGCTTGATACAGGG-TAMRA

 

Sp-Spike-qPCR3

ACGCAATCTAGCAGATGAAGCTATCATGGCGACGTGTTTCATGCAGATATATCGGTAAACGATTTGTTGGCTAAG

 

Sp-spike-probe3

HEX-CATGGCGACGTGTTTCATGCAGATA-BHQ1

Clinical CST

CST_01-M13F

GTAAAACGACGGCCAGCATTCGCATATCGTTTTTG

 

CST_03-M13F

GTAAAACGACGGCCAGCATTCGCACATCGTCTTTG

 

M13wzh1553F

GTAAAACGACGGCCAGACCATTGTCTCTACCTCTCAC

 

M13wzh1553Fst3

GTAAAACGACGGCCAGATGATTGTGTCTACTTCGCAT

 

M13wzh1747R

CAGGAAACAGCTATGACATCAAGGCATAACGACTATCA

 

CST_01-M13R

CAGGAAACAGCTATGACCTGAGCTCTTTTTTTCATGA

 

CST_04-M13R

CAGGAAACAGCTATGACCCGAGCTCTCTTTTTCATGA

 

M13F

GTAAAACGACGGCCAG

 

M13R

CAGGAAACAGCTATGAC

  1. 1used in the general study [8,35].
  2. 2used in the comparison sample set [40].
  3. 3DNA sequence used for internal control of the qPCR.
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BMC Infectious Diseases

ISSN: 1471-2334

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