Figure 3

Evaluation of CD4+ and CD8+ T-lymphocyte proliferation in response to solubleLeishmaniaantigens (SLA) in a patient with mucocutaneous leishmaniasis as manifestation of IRIS. Blood samples were collected before (August 2009) and one day following the conclusion of Amphotericin B and corticosteroid treatment (September 2009). The cell division index was calculated using Flowjo™ software. White and black bars represent the T-cell proliferative responses to Leishmania antigens prior to and after Amphotericin B treatment, respectively. Dashes represent the threshold value with respect to a positive proliferative response (above 0.06 for CD4+ T-cell and above 0.09 CD8+ T-cell subset).