Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae clinical isolates from Cairo, Egypt

Table 1 Susceptibility data of the clinical isolates and the transconjugant

	Zone diameter of inhibition (mm)
Clinical Isolate	CTX	CTX/CLA	CAZ	CAZ/CLA	TET	GEN	AMK	CIP	SXT
Kp4	6	18	18	25	6	8	20	6	ND
Kp8	6	16	19	26	6	8	20	6	ND
Kp15	6	16	18	25	6	8	20	6	ND
ENT	10	20	21	25	20	17	21	30	ND
EC	9	22	15	26	19	19	20	32	6
TcEC^a	11	31	20	32	25	ND	ND	ND	33
EC Na az^R	34	32	29	29	22	23	ND	28	30

Isolates were tested for susceptibility using disk diffusion according to CLSI guidelines [14].
CTX: cefotaxime, CTX/CLA: cefotaxime/clavulanic acid, CAZ: ceftazidime, CAZ/CLA: ceftazidime/clavulanic acid, TET: tetracycline, GEN: gentamicin; AMK: amikacin; CIP: ciprofloxacin, SXT: sulfamethoxazole/trimethoprim,
TcEC: transconjugant carrying plasmid encoding bla _CTX-M-15.
EC Na az^R: recipient strain resistant to sodium azide
^aTransconjugant was tested by PCR experiment for bla _{CTX-M-15-like} using specific primers [16].
ND: Not determined

ISSN: 1471-2334