A representative RT-PCR on total RNA from rat kidneys (A). A representative immunoblotting for PDE3A in the kidney and heart tissue extracts prepared from control and LPS treated group (B). LPS treatment induced 9-fold greater expression of PDE3B mRNA transcript than control. GAPDH was used as a loading control (A). PDE3A was not detected in the kidney tissue either with LPS or without LPS. However, PDE3A was detected in the heart tissue, and LPS caused an increase in its expression in the heart (B).