Regions in the 5'NTR of enterovirus genome examined for the design of RT-LAMP primers. a Schematic view of a model of the secondary structure of 5'NTR of enterovirus genome [22–24]. The region examined for the design of RT-LAMP primers is shown in a box. b Primary and secondary structure of 5'NTR of PV1(Mahoney) genome and RT-LAMP primers used in this study. The structure is based on the model proposed by Pilipenko et al. . The region examined for RT-LAMP primers is shown in boxes on the secondary structure. The numbers on the RT-LAMP primers represent corresponding nucleotide positions on the 5'NTR. For primers that have complimentary sequence to the 5'NTR, the numbers are shown in parenthesis. c Scheme of RT-LAMP procedure examined in this study.