ELISA for total IgG antibody levels from acute (AC), indeterminate (IND) and cardiac chronic (CCC) chagasic patients and healthy individuals (HD) against
lysate (2A), KMP-11 (2B), truncated
HSP-70 (TcHSP-70T) (2C), and
HSP-70 (2D) recombinant proteins. Values are given as optical densities at 405 nm. The dotted line represents the cut-off values based on the mean of healthy individual values plus 3 standard deviations. Horizontal lines on each group represent mean and standard deviation values, the mean being the larger one. Statistically significant differences among chagasic groups are represented by an asterisk.