Table 1 Oligonucleotide primers and hybridization probes used in this study
Name | Function | Sequence 5' - 3' |
|---|---|---|
gbsU3F | Forward primer | GACAGGCATTATGAGGTA |
gbsU4R | Reverse primer | GCTAATATATTTGTCTACAAC |
CompF | Forward composite primer for IAC generation | GACAGGCATTATGAGGTAATACCCAACTTGGAATG |
CompR | Reverse composite primer for IAC generation | GCTAATATATTTGTCTACAACTCTTCACCAGAATAAAATTG |
tmUF | Universal bacterial ssrA forward primer | GGGG(A/C)(C/T)TACGG(A/T)TTCGAC |
tmUR | Universal bacterial ssrA reverse primer | GGGA(A/G)TCGAACC(A/G)(C/G)GTCC |
f1GBS-Flu | GBS-specific hybridization probe | TTGCGTTTTGCTAGAAGGTCTTA - Flu |
f2GBS-LC640 | GBS-specific hybridization probe | LC640- TATCAGCAAACTACGTTTGGCT - Ph |
ALS1-Flu | IAC-specific hybridization probe | TGAATGTATCCCCTGGA - Flu |
ALS1-LC705 | IAC-specific hybridization probe | LC705 - TGGCACTGGTACCATCTAA - Ph |