Phenotypic and molecular characterization of Staphylococcus aureus isolates expressing low- and high-level mupirocin resistance in Nigeria and South Africa

Table 1 Characterization (antibiogram, PCR-RFLP of the coagulase gene and PFGE) of low and high-level mupirocin resistant S. aureus isolated in Nigeria and South Africa

Strain No	Antibacterial resistance patterns	MUP MIC (mg/L)	mupA gene	Coagulase gene (± 20 bp)	PCR-RFLP Coagulase gene (bp)	PFGE type
A1	PEN, OX, GN, ERY, CHL, TET, TS, CIP, MU (L), Hg	12	-	800	324, 405	A
A2	PEN, OX, GN, TET, TS, RF, MU (L)	24	-	650	81, 567	B
A3	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	16	-	850	324, 405	A
A4	PEN, OX, GN, TET, TS, RF, MU (L), Cad, Hg	24	-	650	81, 567	B
A5	PEN, OX, GN, ERY, CHL, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	12	-	800	324, 405	A1
A6	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	8	-	800	81, 324, 405	A
A7	PEN, OX, GN, ERY, TET, TS, RF, MU (L), Cad	24	-	650	81, 567	B1
A8	PEN, OX, GN, ERY, CHL, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	8	-	800	81, 324, 405	A
A9	PEN, OX, GN, ERY, TET, TS, RF, MU (L), Cad, Hg	24	-	650	81, 567	B2
A10	PEN, OX, GN, ERY, CHL, TET, TS, CIP, MU (L), Hg	12	-	800	81, 324, 405	A
A11	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	8	-	800	81, 324, 405	A
A12	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Hg	12	-	850	81, 324, 405	A2
A13	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Hg	8	-	800	81, 324, 405	A1
A14	PEN, OX, GN, ERY, TET, TS, CIP, MU (L), Cad, Pi, Hg, Eb	12	-	800	81, 324, 405	A
A15	PEN, OX, GN, ERY, TET, TS, RF, MU (H), Cad	>1024	+	650	81, 567	B3
P1929	TM, TET, MU (H), Cad	>1024	+	750	243, 486	C
35 IBA	TET, MU (H), Cad	>1024	+	800	81, 162, 567	D

KEY. PEN – Penicillin, OX – Oxacillin, GN – Gentamicin, ERY – Erythromycin, CHL – Chloramphenicol,
TET – Tetracycline, TS – Trimethoprim, RF – Rifamipicin, CIP – Ciprofloxacin,
MU (H) – High-level mupirocin resistance, MU (L) – Low-level mupirocin resistance
Heavy metals – Cad: Cadmium acetate; Hg: Mercuric chloride Nucleic acid binding compounds – Eb: Ethidium bromide; Pi: Propamidine isethionate

ISSN: 1471-2334