Expression of TGF-β1 and S100B in the brains of
infected mice. (A) Protein levels of TGF-β1 and S100B in the brains of T. canis infected mice from 3 dpi to 8 wpi assessed by Western blotting. β-actin was used as a loading control. (B) Relative times were generated by comparing intensities between infected and uninfected mice. The error bars indicate the standard deviations and the superscript indicates significant differences from control; #
P < 0.001. (C) Expression levels of TGF-β1 and S100B in the brains of infected mice from 3 dpi to 8 wpi were examined by RT-PCR. GADPH was used as a reaction control. (D) The relative amounts of TGF-β1 and S100B mRNA were calculated based on the optical density relative to that of the GAPDH. The error bars indicate the S.D. and the superscripts represent significant differences from the control; *P < 0.05, #
P < 0.001. Three to eight mice per group were examined.