Distribution of total amino acid charge of the V1-V2-V3 fragment per study group. Distribution of the nett charges of the V1-V2-V3 fragment in children with severe anaemia (cases, Hb<5.0 g/dL) as compared to those without severe anaemia (controls). The distribution is expressed as a percentage of the total number of fragments analysed per study group (Controls: n = 26, Cases n = 25). Error bars express 95% Confidence intervals. p = 0.36.