Figure 1
Entry of H. influenzae into A549 cells. Cell cultures (1 × 105 cells/well) were infected for 1 h at 37°C with 1 × 107 CFU of YS001, YS002, YS005, YS008, YS009, or YS010. At 1 h after infection, the cell culture medium was exchanged for fresh medium containing GEN (100 μg/ml). One hour later, the surviving intracellular bacteria were enumerated by plating. Data represent the means ± SD of three experiments with triplicate assays. ND, not detected.