SDS-PAGE Western blot of anthrax infected rabbit plasma showing PA
just prior to death. Lanes 1 to 4 were stained with anti-PA20 specific MAb and lanes 5 to 8 stained with anti – PA63 specific MAb. Lanes 1 (arrow points to PA17) and 5 were loaded with the LIST PA17 at 5 mg/ml. Lanes 2 and 6 were left blank to minimize cross contamination. Lanes 3 (arrow points to PA20) and 7 (arrow points to PA63) were loaded with 1:20 dilution of plasma from Ames strain challenged rabbit #41 just prior to death. Lanes 4 (arrow points to PA83) and 8 (arrow points to PA83) were loaded with purified PA83 @ 10 mg/ml.