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Table 3 Confirmation of B. cenocepacia gene expression by Real Time PCR

From: Gene expression changes linked to antimicrobial resistance, oxidative stress, iron depletion and retained motility are observed when Burkholderia cenocepaciagrows in cystic fibrosis sputum

  Real-time PCR fold change using delta delta CT method
Gene expression determined from
(infection; clinical condition):
BCAL0270
(ferric reductase
transmembrane component)
BCAM2753
(ohrgene, organic
hydroperoxide resistance protein)
BCAL1107
(oxidoreductase)
BCAM1676
(nitrite/sulfite
reductase)
Microarray dataset
(Sputum 1, 2, and 3; see below)
↑ 36 ↑ 8 ↑ 66 ↑ 20
Sputum 2
(B. cenocepacia; stable)
↑ 160 ↑ 100 ↑ 914 ↑ 97
Sputum 3
(B. cenocepacia; exacerbation)
↑ 65 ↑ 40 ↑ 299 ↑ 4
Sputum 4
(B. cenocepacia; stable)
↑ 25 ↑ 22 ↑ 235 -
Sputum 5
(P. aeruginosa; exacerbation)
↑ 22 ↑ 71 ↑ 189 -
Sputum 6
(P. aeruginosa; stable)
↑ 3 ↑ 3 ↑ 12 ↑ 94
Average for sputum growth ↑ 55 ↑ 47 ↑ 330 ↑ 65
Fold change in minimal media with:     
No FeSO 4 ↑ 2 -   
0.1% H 2 O 2 - ↑ 5   
0.15% H 2 O 2 - ↑ 3   
0.002% t-butyl hydroperoxide - ↑ 2