Resistance of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisto nitric oxide correlates with disease severity in Tegumentary Leishmaniasis

Table 3 Thymidine incorporation assay of susceptible L (L.) amazonensis

4 × 10⁵ L. (L.) amazonensis and L. (V.) braziliensis promastigotes in HBSS pH 5.0 were exposed to increased NaNO₂ concentrations (0.25–16 mM) for 4 hours. The Leishmania were then washed, distributed in parasite growth medium in the presence of 1 μci/ml of ³H-TdR. After 20 hours of incubation, the incorporation of Thymidine was measured in Beta scintillation counter (CPM). Data are mean ± SD of NO^•-resistant (Table 2) or NO^•-susceptible (Table 3) isolates of L. (L.) amazonensis and L. (V.) braziliensis

ISSN: 1471-2334