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Table 3 Thymidine incorporation assay of susceptible L (L.) amazonensis

From: Resistance of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisto nitric oxide correlates with disease severity in Tegumentary Leishmaniasis

L (L.) amazonensis NaNO2 Concentration (mM)
  0 0.25 0.5 1 2 4 8 16
8653 1227 1244 513 262 174 102 81 61
9667 1364 1595 1255 1078 200 65 75 54
L. (V.) braziliensis NaNO2 Concentration (mM)
  0 0.25 0.5 1 2 4 8 16
13323 2339 3217 1636 215 92 88 76 83
Lb-001 2213 2368 1978 1319 830 482 268 242
13352 1879 1227 1318 870 868 474 326 196
13468 1626 1796 169 78 73 85 89 97
  1. 4 × 105 L. (L.) amazonensis and L. (V.) braziliensis promastigotes in HBSS pH 5.0 were exposed to increased NaNO2 concentrations (0.25–16 mM) for 4 hours. The Leishmania were then washed, distributed in parasite growth medium in the presence of 1 μci/ml of 3H-TdR. After 20 hours of incubation, the incorporation of Thymidine was measured in Beta scintillation counter (CPM). Data are mean ± SD of NO-resistant (Table 2) or NO-susceptible (Table 3) isolates of L. (L.) amazonensis and L. (V.) braziliensis