Sensitivity and specificity of monoclonal and polyclonal immunohistochemical staining for West Nile virus in various organs from American crows (Corvus brachyrhynchos)

Table 2 Average value assigned to each organ for staining intensity (ease of identification) and percentage of positive staining cells by tissue type and IHC staining method

	Monoclonal Antibody		Polyclonal Antibody
	Ease of identification at 2.5×^a	Percentage of positive cells^b	Ease of identification at 2.5×^a	Percentage of positive cells^b
Heart	1.55	1.00	1.62	1.21
Kidney	2.28	1.08	2.08	1.18
Brain	1.60	1.00	1.17	1.00
Spleen	2.50	1.70	2.57	1.86
Liver	2.10	1.40	2.33	1.80
Lung	2.70	1.30	2.46	1.38
Small intestine	2.67	1.67	2.94	2.00

^a Ease of identification:
1 = positive staining could not be identified at 2.5× (but was identified at higher magnification); 2 = faint positive staining could be identified at 2.5×; 3 = positive staining was easily identified at 2.5×
^b Percentage of positive cells:
1 = less than 20% of the cells stained positively; 2 = 20% to 80% of the cells stained positively; 3 = greater than 80% of the cells stained positively

ISSN: 1471-2334