Figure 2

Western blotting using Bartonella antigen of a patient with IE. Western blotting was performed with the first serum sample from May 2005 at a 1:200 dilution. Molecular masses (in kilodaltons) are given on the left. A through C: Serum was analyzed by using B. quintana (lane 1), B. henselae (lane 2), B. elizabethae (lane 3), B. vinsonii subsp. Berkhoffii (lane 4) and B. alsatica (lane 5) antigens. (A) Untreated serum. (B) B. quintana-adsorbed serum. Antibodies to the B. henselae remained. (C) B. henselae-adsorbed serum. All antibodies were removed.