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Table 1 Reproducibility of real-time PCR with low-, intermediate-, and high-titre plasma pools.

From: Relationship between pp65 antigenemia levels and real-time quantitative DNA PCR for Human Cytomegalovirus (HCMV) management in immunocompromised patients

 

PLASMA POOLS

PRECISION

Low-titre

Intermediate-titre

High-titre

 

Mean value (copies/ml)

CV%

Mean value (copies/ml)

CV%

Mean value (copies/ml)

CV%

Intra-assay

3,162

27.8

50,119

23.5

100,000

21.2

Inter-assay

3,981

28.8

63,096

23.3

107,000

26.7