Figure 4

Supernatants from A549 cells treated with high- and low-binding S. pneumoniae were evaluated with a RayBio^® Human Cytokine Antibody Array III, which detects 42 different human cytokines. 1A and 2A, positive control; 3A and 4A, negative control; 5A, ENA-78; 6A, GCSF; 7A, GM-CSF, 8A, GRO; 9A, Gro-α; 10A, I-309; 11A, IL-1α; 12A, IL-1β; 1B, IL-2; 2B, IL-3; 3B, IL-4; 4B, IL-5; 5B, IL-6; 6B, IL-7; 7B, IL-8; 8B, IL-10; 9B, IL-12; 10B, IL-13; 11B, IL-15; 12B, IFN-γ; 1C, MCP-1; 2C, MCP-2; 3C, MCP-3; 4C, MCSF; 5C, MDC; 6C, MIG; 7C, MIP-1; 8C, RANTES; 9C, SCF; 10C, SDF-1; 11C, TARC; 12C, TGF-β; 1D, TNF-α; 2D, TNF-β; 3D, EGF; 4D, IGF-1; 5D, angiogenin; 6D, oncostatin M; 7D, thrombopoietin; 8D, VEGF; 9D, PDGF; 10D, leptin; 11D, negative control; 12D, positive control. The positive result for IL-8, in position 7B, with negative results for all other cytokines, was observed for A549 cells treated with both high- and low-binding bacteria. Experiment replicated with four different high-binding, and four different low-binding, S. pneumoniae strains.