Flowchart Depicting Modified Protocols for LightCycler Based
Allelic Typing. Modified flowchart of protocols from Mäkinen et al. detailing the updated procedure for LightCycler based allelic typing of the B. pertussis prn gene. Electrophoretic sizing of all QH8F-QH2R PCR products from the FRET HybProbe assay in addition to melting curve analysis enables determination of new alleles detected since publication of the original method.