HIV-1 subtype A infection in a community of intravenous drug users in Pakistan

BMC Infectious Diseases

Table 1 List of primers employed in the study.

Primer	Sequence
PC03	ACACAACTGTGTTCACTAGC
PC04	CAACTTCATCCACGTTCACC
JA9AE	CACAGTACAATGCACACATG
JA9B	CACAGTACAATGTACACATG
JA12A	GCAATAGAAAAATTCTCCTC
JA12B	ACAGTAGAAAAATTCCCCTC
JA10UB	CTGTTAAATGGCAGTCTAGC
JA10UC	CTGTTAAATGGTAGTCTAGC
JA10UG	CTGTTAAATGGCAGTTTAGC
JA11LAE	AATTTCTAGATCCCCTCCTG
JA11LB	AATTTCTGGGTCCCCTCCTG
JA11LC	AATTTCTAGGTCCCCTCCTG
JA11QA	CCCCTCCTGAGGAGTTAGCA
JA11VB	CACAATTAAAACTGTGCATTACAA
JA11XC	TTGTTTTATTAGGGAAGTGTTC
JA11YE	AAATTCCCCTCTACAATTAAAATGA
Primers used in gag HMA
HIG777	TCACCTAGAACTTTGAATGCATGGG
H1P0202	CTAATACTGTATCATCTGCTCCTGT
H1Gag1584	AAAGATGGATAATCCTGGG
g17	TCCACATTTCCAACAGCCCTTTT

PC03/PC04 primers for β-Globin, JA9AE, JA9B are the upstream primers and JA12A, JA12B are the downstream primer for the first round PCR. JA10UB, JA10UC and JA10UG are the inner upstream primers; JA11LAE, JA11LB and JA11LC are the inner downstream general primers. JA11QA, JA11VB, JA11XC and JA11YE are the inner downstream subtype specific primers for subtypes A, B, C and CRF01-AE, respectively.
Primers for gag HMA are relative to HIV-1 group M strain ELI (GenBank accession number K03454)

ISSN: 1471-2334