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Table 2 In vitro antibacterial activity of purified phospholipase A2 enzymes from snake venoms.

From: In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei

Phospholipase A2
enzymes (PLA2s)
Snake species (Scientific name) Mol. wt.(kDa) Conc. (mg/mL) Micro-organisms
     B. pseudomallei (Strain KHW) B. pseudomallei (Strain TES)
Crotoxin A (CA) Crotalus durissus terrificus 23.5 0.5 mg/mL - -
Crotoxin B (CB) Crotalus durissus terrificus 23.5 0.5 mg/mL 24.8 ± 0.089 27.6 ± 0.133
Ammodytoxin A Vipera ammodytes ammodytes 13.8 0.5 mg/mL - -
Mojave toxin Crotalus scutulatus scutulatus 23.5 0.5 mg/mL - -
β-Bungarotoxin Bungarus multicinctus 20.5 0.5 mg/mL - -
Taipoxin Oxyuranus scutellatus scutellatus 45.6 0.5 mg/mL -  
Mulgatoxin Pseudechis australis 13.2 0.5 mg/mL 20.5 ± 0.075 22.7 ± 0.117
Daboiatoxin (DbTx) Daboia russelli siamensis 15.0 0.5 mg/mL 24.8 ± 0.103 26.2 ± 0.121
Bee venom PLA2 Apis mellifera 19.0 0.5 μg/mL 18.3 ± 0.089 20.4 ± 0.075
  1. *The values given represent a venom inhibition zone in mm, including the 7 mm diameter of the disc, after 24 h incubation. The bacterial inoculum per plate contained 3.2 × 108 cfu/mL forming units which were spread onto the TS agar surface with sterile cotton swap. Sterile paper discs (7 mm diameter) were placed onto the TS agar surface and 20 μL of enzymes (0.5 mg/ml concentration) added, Control (0); No activity (-).