Figure 5
Effect of an iNOS inhibitor and of a NO donor on DENV-Ag expression after cell infection. Figures 5A and 5B – Effect of iNOS inhibitor – NGMLA on monocyte infection. Adherent PBMLs were incubated with either Mock C6/36 cell supernatant or DENV inoculum for 4 days in presence or absence of 400 μM NGMLA. Figure 4A shows histogram with profile of infected monocytes after NGMLA treatment and labelling controls. Figure 4B shows percentages of DENV-Ag+ in gated monocytes obtained by flow cytometry. Figures 5C and 5D -. Effect of NO donor – SNP, on C6/36 cell infection. C6/36 cells were incubated with DENV for 2 days in absence or presence of 10 or 100 μM SNP. Figure 4C shows a histogram with profiles of infected C6/36 cells obtained by FACS from a culture treated with 10 μM SNP or controls. The histogram gates have been made where ≥99% than cells in the cell control are excluded. Figure 4Dshows percentages of DENV-Ag+ cells calculated from ungated cells after labelling with antibody to DENV-Complex and anti-mouse IgG-PE. In histograms x-axis represent mean of fluorescence intensity and y-axis represent percentage of maximum cell number counts. Cells were labelled with antibody to DENV-Complex followed by anti-mouse IgG-PE. Average ± SEM are represented from samples set in triplicates. * P < 0.05 in Student's T-test showing difference in DENV infection after treatment. Data are obtained from one representative experiment out of two for each cell type using two different monocyte donors.