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Table 3 DEN-2 Tonga/74 is poorly infectious for Aedes aegypti fed an infectious bloodmeal, and the Δ30 mutation does not decrease infectivity for Toxorynchites amboinensis.

From: Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2

  A. aegypti (oral infection) T. amboinensis (intrathoracic inoculation)
Virus Dose (log10PFU)a No. tested % Infectedb Dose (log10PFU)c No. tested % Infectedd
    Midgut Head    
DEN-2 Tonga/74 2.8 20 0 0 -- -- --
rDEN-2 3.5 18 0 0 2.9 5 100
      1.9 12 83
      0.9 8 63
      -0.1 7 14
      -1.1 7 0
        MID50 = 0.9
rDEN2Δ30 3.5 22 0 0 2.7 8 100
      1.7 7 100
      0.7 6 83
      -0.3 7 43
      -1.3 4 50
      -2.3 5 0
        MID50 = -0.2
  1. a Amount of virus ingested, assuming a 2 ul bloodmeal.
  2. b Percentage of mosquitoes with antigen detectable by IFA in midgut or head tissue prepared 21 days after oral infection.
  3. c Amount of virus present in 0.22 ul inoculum.
  4. d Percentage of mosquitoes with antigen detectable by IFA in head tissue prepared 14 days post-inoculation.