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Table 1 Comparison of the absorbance values with different denaturing washing conditions in the VZV IgG avidity assay.

From: A new method for determination of varicella-zoster virus immunoglobulin G avidity in serum and cerebrospinal fluid

VZV infection status

3 × 5 min 5 M urea

1 × 3 min 4 M urea H2O2

 

Abs (ref)1

Abs (denat)2

Avidity

Abs (ref)

Abs (denat)

Avidity

acute

2.221

1.208

54.4%

2.359

0.546

23.2%

acute

1.829

0.676

37.0%

1.666

0.300

18.0%

acute

1.982

0.457

23.1%

1.869

0.251

13.4%

past

1.487

1.387

93.3%

1.448

0.850

58.7%

past

1.190

0.830

69.7%

1.072

0.580

54.1%

past

2.020

1.671

82.7%

1.929

0.984

51.0%

  1. 1absorbance value in EIA without denaturing washing step
  2. 2absorbance value in EIA with denaturing washing step