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Table 2 Invasion, adhesion and cytotoxicity of different bacterial strains at low and high MOI. Invasion: RT112 cells incubated for 2 h, 37°C with bacteria prior to addition of 100 μg/ml gentamicin; adhesion was measured by the MTT assay as described; cytotoxicity was determined at MOI 2 and 10 after 2 h of incubation.

From: Serratia marcescens internalization and replication in human bladder epithelial cells

  MOI 2 MOI 10  
Invasion    
S. marcescens W225 5669 ± 213 4234 ± 877
S. marcescens SM001 64 ± 23 13986 ± 1322
SM001 pRSC01 5011 ± 197 3698 ± 762
E. coli BL21 [pRO3] 2 ± 1.3 46 ± 12
Relative adhesion   OD492 [%]
S. marcescens W225 not detected 0.60 ± 0.059 100 ± 9.9
S. marcescens SM001 not detected 0.59 ± 0.076 99.6 ± 12.8
SM001 pRSC01 not detected 0.58 ± 0.112 96.6 ± 18.8
E. coli BL21 [pRO3] not detected 0.0 ± 0.017 0 ± 2.8
Absolute cytotoxicity [%]    
S. marcescens W225 16 ± 2.4 90 ± 1.8
S. marcescens SM001 0 7 ± 0.4
SM001 pRSC01 19 ± 3.2 95 ± 4.3
E. coli BL21 [pRO3] 0 22 ± 0.4