Table 2 Invasion, adhesion and cytotoxicity of different bacterial strains at low and high MOI. Invasion: RT112 cells incubated for 2 h, 37°C with bacteria prior to addition of 100 μg/ml gentamicin; adhesion was measured by the MTT assay as described; cytotoxicity was determined at MOI 2 and 10 after 2 h of incubation.
From: Serratia marcescens internalization and replication in human bladder epithelial cells
| Â | MOI 2 | MOI 10 | Â |
|---|---|---|---|
Invasion | Â | Â | Â |
S. marcescens W225 | 5669 ± 213 | 4234 ± 877 | |
S. marcescens SM001 | 64 ± 23 | 13986 ± 1322 | |
SM001 pRSC01 | 5011 ± 197 | 3698 ± 762 | |
E. coli BL21 [pRO3] | 2 ± 1.3 | 46 ± 12 | |
Relative adhesion | Â | OD492 | [%] |
S. marcescens W225 | not detected | 0.60 ± 0.059 | 100 ± 9.9 |
S. marcescens SM001 | not detected | 0.59 ± 0.076 | 99.6 ± 12.8 |
SM001 pRSC01 | not detected | 0.58 ± 0.112 | 96.6 ± 18.8 |
E. coli BL21 [pRO3] | not detected | 0.0 ± 0.017 | 0 ± 2.8 |
Absolute cytotoxicity [%] | Â | Â | Â |
S. marcescens W225 | 16 ± 2.4 | 90 ± 1.8 | |
S. marcescens SM001 | 0 | 7 ± 0.4 | |
SM001 pRSC01 | 19 ± 3.2 | 95 ± 4.3 | |
E. coli BL21 [pRO3] | 0 | 22 ± 0.4 | |