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Table 1 Bacteria, plasmids, and epithelial cells used in this study

From: Serratia marcescens internalization and replication in human bladder epithelial cells

Strains/plasmids/cell lines relevant genotype source or reference
E. coli   
BL21 [DE3] F-, hsdS gal, lysogenic for phage λ carrying the phage T7 RNA polymerase gene under lacUV5 control [36]
SM10[λpir] thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::mu, Kmr, λpir [28]
HB101 ara leu pro lac gal rpsL supE44 xyl mtl recA hsd9 (hsdR hsdM) Stratagene
S. marcescens   
W225 human isolate [31]
SM001 W225 shlB/shlA::kan, nonhemolytic this study
SM011 W225 shlB/shlA::kan, nonhemolytic this study
VA 15854 human isolate [31]
W1128 human isolate [30]
ATCC25419 human isolate [31]
CDC 04:H4 human isolate [31]
Other strains   
Proteus mirabilis VI human isolate [34]
Pseudomonas aeruginosa PAO1 S. Lory
Plasmids   
pGP704 Apr; ori R6 K, mob RP4, MCS of M13tg131 [13]
pRK2013 conjugative IncP helper plasmid ATCC 37159
pMMB67EH multi-host cloning and expression vector ATCC 37622
pRSC01 pMMB67EH shlB shlA this work
pUC4-K Apr, Kmr; MCS [37]
pRSM01 Apr, Kmr; ori R6 K, mob RP4, MCS of M13tg131 this work
pRSM02 pRSM01, 1.8-kb SphI fragment of pRO2 [shlB'/shlA'] this work
pRSM03 pRSM02, SgrAI deletion [shlB/shlA, Δ bp 1544–2285] this work
pRO3 pT7-5 shlB shlA [28]
pRO2 pT7-5 shlB shlA, Δ aa 256–1578 [28]
Epithelial cells   
RT112 human bladder epithelial carcinoma cell line MPI Tübingen
HEp-2 human larynx epidermoid carcinoma cell line ATCC CCL23
HeLa human cervix epitheloid carcinoma cell line ATCC CCL 2
Hec1-B human endometrial adenocarcinoma cell line ATCC HTB 113