Figure 1

HBD-1 and HBD-2 specific RT-PCR of normal renal parenchyma and renal tissues with chronic infection. HBD-1 (Fig. 1a) specific RT-PCR (PCR product: 184 bp), HBD-2 (Fig. 1a) specific RT-PCR (PCR product: 255 bp). Samples were separated by 2% agarose gel electrophoresis and stained with ethidium bromide. Representative data from a panel of 30 specimens are presented. Lanes 1 to 10 represent renal tissue extracts with chronic infection. Lanes 11–20 show RT-PCR results of normal renal tissue extracts from tumor-bearing organs. Outer lanes (M) represent a 100 bp-ladder molecular-weight marker. The control reaction for correct cDNA synthesis in all samples was performed as RT-PCR for GAPDH (glyceraldehyde-3-phosphate dehydrogenase) yielding a 359 bp product. Correct positive and negative controls including amplification without reverse transcription or cDNA sample replaced by sterile water were performed with each original experiment but are not shown due to limited gel space.