Development of real-time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV-8 lytic and latent genes

Table 2 Sequences of the primers and beacons for each of the assays

	Primer P1 Anti-sense primer	Primer P2 Sense primer	Beacon Stem-loop in lowercase italics
ORF73	AG AGA CAA TAC ACA TAT ACA CAA TAA G	GAA AGG ATG GAA GAC GAG ATC CA	gca cgc AGG AGT AAA GGC AGG CCC CGT GTC gc g tgc
vGCR	AA CGA GGT TAC TGC CAG ACC CAC GT	CAG GCG GAA GGT AAG GGG GGT GA	gca cgc TGA TTG TTG CTG TGG TGC TGC T gcg tcg
vBcl-2	AA GCG AAA CCA CTG GGG TCC GAT TG	GTG AGA TTT CAC AGC ACC ACC GGT A	gca cgc TGA CCT TTG GCA GTT TTG TGG CC gcg tgc
vIL-6	AG AAC ATA AAA CGA AGC AAA GTG TCT CA	GGA AAA TCA GTG ATA AAC GTG GA	gca cgc GGA AGA GCT CAA TAA GCT GAC TA gcg tgc
U1A	AG AGG CCC GGC ATG TGG TGC ATA A	CAG TAT GCC AAG ACC GAC TCA GA	cgt acg AGA AGA GGA AGC CCA AGA GCC A cgt acg

The 3' anti-sense primer is elongated with T7-promotor recognition sequence: AAT TCT AAT ACG ACT CAC TAT AGG G

ISSN: 1471-2334