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BMC Infectious Diseases

Open Access

Assessment of bacteriophage activity against local strains of Enterococcus and Pseudomonasin Romania

  • Alina Cristina Neguț1, 2Email author,
  • Oana Săndulescu1, 2,
  • Anca Streinu-Cercel1, 2,
  • Zemphira Alavidze3,
  • Ioana Berciu1, 2,
  • Veronica Ilie2,
  • Magdalena Lorena Andrei2,
  • Dana Mărculescu2,
  • Mircea Ioan Popa1 and
  • Adrian Streinu-Cercel1, 2
BMC Infectious Diseases201414(Suppl 7):O19

https://doi.org/10.1186/1471-2334-14-S7-O19

Published: 15 October 2014

Background

Enterococcus faecium and Pseudomonas aeruginosa are part of the ESCAPE pathogens, which have the ability to “escape” the currently available therapeutic options. For these germs alternatives are needed, as is the case of bacteriophage treatment.

Methods

In this study we used a bacteriophage testing kit containing 4 types of Georgian products: PYO, INTESTI (Eliava BioPreparations, Tbilisi) and PHAGYO, PHAGESTI (JSC "Biochimpharm", Tbilisi) to test the strains of Pseudomonas spp. and Enterococcus spp. isolated and stored from patients treated in the Adults II ward of the National Institute for Infectious Diseases “Prof. Dr. Matei Balş”, Romania during April 2013 – July 2014.

Results

We identified 9 strains of Enterococcus (7 E. faecalis, 1 E. avium and 1 E. faecium) and 9 strains of Pseudomonas aeruginosa. The strains had been isolated mostly from cutaneous wounds (3/9) for Enterococcus spp. and from urine (5/9) for P. aeruginosa. For Enterococcus spp. the rate of susceptibility to PYO phages was 33.33% (3/9), to INTESTI 55.56% (5/9), to PHAGYO 44.44% (4/9) and to PHAGESTI 0% (0/9). We tested the Enterococcus ATTC, and it displayed susceptibility to PYO, INTESTI and PHAGYO.

For Pseudomonas spp. the rate of susceptibility to PYO phages was of 66.67% (6/9), INTESTI 88.89% (8/9), PHAGYO 55.56% (5/9), PHAGESTI 44.44% (4/9). We tested the Pseudomonas ATTC, and it displayed susceptibility to all the bacteriophage products tested.

Performing the ANOVA test for Enterococcus spp. we identified a statistically significant correlation between susceptibility to ampicillin vs. PYO phages (p=0.034) and vs. INTESTI phages (p=0.024). For P. aeruginosa a correlation was identified between susceptibility to ceftazidime and PYO phages (p=0.029).

Conclusion

Despite the fact that PYO and PHAGYO do not contain phages for Enterococcus spp., their activity against it was similar to that of INTESTI. PYO and PHAGYO showed activity on Enterococcus ATCC, too. For P. aeruginosa the rate of susceptibility was very high for phages, and quite low for almost all antibiotics tested. We intend to develop further studies for testing a higher number of strains and for assessing a potential synergy for co-administration of antibiotics and bacteriophages.

Declarations

Acknowledgements

1. This paper is partly supported by the Sectorial Operational Programme Human Resources Development (SOPHRD), financed by the European Social Fund and the Romanian Government under the contract number POSDRU 141531.

2. This paper is partly supported by the Carol Davila University of Medicine and Pharmacy, Young Researchers Grant, no 28341/2013.

Authors’ Affiliations

(1)
Carol Davila University of Medicine and Pharmacy
(2)
National Institute for Infectious Diseases "Prof. Dr. Matei Balş"
(3)
Phage Therapy Center Tbilisi

Copyright

© Neguț et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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