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  • Poster presentation
  • Open Access

Emerging MDR producing ESBL among Diarrhoegenic Esherichia coli from paediatric patients

BMC Infectious Diseases201414 (Suppl 3) :P70

  • Published:


  • Beta Lactam Antibiotic
  • ESBL Production
  • Clinical Laboratory Standard Institute
  • Hospital Sample
  • Beta Lactamase


The beta lactam antibiotics are predominant therapeutic agents against a number of infectious diseases. The production of the enzyme beta lactamase by microbes which hydrolyses the beta lactam antibiotics is an important mechanism of resistance. This study aimed to determine prevalence, phenotypic patterns, and ESBL-production status both phenotypic and genotypic for the CTX –M type ESBL in Escherichia coli (E.coli) isolated from Diarrhoeic children.


Two hundred thirty non repetitive stool samples were collected from 2 hospitals, one private and other government hospital in Delhi, NCR. E.coli was confirmed by 16S rRNA analyses. Antibiotic susceptibility and phenotypic ESBL production were studied by disc diffusion and double disk synergy tests according to Clinical Laboratory Standards Institute guidelines. PCR was performed for the molecular detection of the CTX-M type ESBL.


Out of 230, 215 were confirmed to be E.coli. Phenotypically 72.5% (156) showed the presence of ESBL of which 75% (79) in private hospital samples and 70% (77) in government hospital samples. PCR based molecular detection confirmed the presence of the CTX-M gene among 65.6% (141) of the samples.


The high trends in the ESBL presence are alarming and urge means to cope with such ESBL E.coli strains. It being a commensal raises concern of the resistance gene transfer from pathogenic bacteria, especially in children due to common diarrhoeal episodes among them. The paediatric population is at a higher risk in case of multidrug resistance due to further limitation in therapeutic options and constrains in prescription of harsher antibiotics.

Authors’ Affiliations

Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India


© Pinto et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.