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BMC Infectious Diseases

Open Access

Prevalence of SCCmec types among Methicillin resistant Coagulase negative Staphylococci isolated from HIV patients in Chennai, South India

  • Murugesan Saravanan1,
  • Betsy S Dass1,
  • Sankar Baby Abirami1,
  • Jaya Kumar Suriakumar2, 3 and
  • Padma Krishnan1Email author
BMC Infectious Diseases201414(Suppl 3):P51

https://doi.org/10.1186/1471-2334-14-S3-P51

Published: 27 May 2014

Background

Methicillin resistant Coagulase negative staphylococci (MRCoNS) are opportunistic pathogens among HIV-AIDS patients and serve as a large reservoir of Staphylococcal Cassette Chromosome mec (SCCmec) that carries mecA. Very little is known about the prevalent SCCmec types among MRCoNS from HIV patients and its molecular epidemiology. Hence, this study was aimed at determining the distribution of SCCmec elements amongst MRCoNS isolates from HIV infected patients from Chennai, South India.

Methods

52 clinically significant MRCoNS isolates from HIV patients were included in this study. Following speciation, isolates were subjected to antibiotic susceptibility testing and screening for methicillin resistance using cefoxitin disc (30µg) and confirmed by mecA gene PCR. The presence of SCCmec types (I - V) was determined using multiplex PCR.

Results

Of the 52 isolates, S. haemolyticus (n =29, 56%) was predominant followed by S. epidermidis (n =13, 25%), S. capitis (n=4, 7%), S. lugdunensis (n =3, 6%) and S. hominis, (n =3, 6%). Highest resistance was shown towards ciprofloxacin (44%) followed by erythromycin (42%) and ofloxacin (39%). SCCmec typing revealed type I (n=26, 50%) to be predominant followed by type V (n=10, 19%), type III (n=7, 14%), type IV (n=4, 7%) and type I & V (n=2, 4%). Three (6%) isolates were nontypeable and type II was absent.

Conclusion

The MRCoNS isolates carried genetically diverse SCCmec elements including types I, V, III, IV and I and V in combination with SCCmec type I being predominant among HIV patients, which may be attributed to majority of patients being hospitalized.

Authors’ Affiliations

(1)
Dr. ALM PG IBMS, University of Madras
(2)
Govt. Hospital of Thoracic Medicine
(3)
Institute of Microbiology, Madurai Medical College

Copyright

© Saravanan et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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