Skip to main content


HIV-1 reverse transcriptase inhibitory activity of Aerva lanata plant extracts

  • 883 Accesses

  • 3 Citations


HIV-1 reverse transcriptase (HIV-1 RT) is an essential enzyme for the replication cycle of HIV. HIV-1 RT inhibitors have been extensively investigated for their anti-HIV properties. However, emergence of HIV drug resistance and side effects are the main reasons for failure of anti-HIV therapy. The aim of the present study was to evaluate the HIV reverse transcriptase inhibitory activity of Aerva lanata plant extracts.


Extracts were prepared from dried roots in different solvents. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts among PBMCs by MTT assay. HIV-1 RT inhibition activity of the all solvent extracts of A. lanata was determined by a HIV-1 Reverse Transcriptase activity assay.


All the five solvent crude extracts of A. lanata were non cytotoxic up to 0.75 mg/mL concentration in PBMCs. Chloroform and methanol extracts shows highest inhibition of recombinant HIV RT (91% and 89% respectively) at 1 mg/mL concentration. This strong inhibitory effect was confirmed by their IC50. More than 50% inhibition of HIV RT shows from 0.03 to 1 mg/mL concentrations of all extracts.


Experimental results thus suggested that the A. lanata plant extracts which have been tested in the present study exert their anti-HIV activity via inhibition of HIV Reverse Transcriptase activity. However, in order to assess the usefulness of this herb, it is necessary to isolate the active principle (s) from the crude extracts.

Author information

Correspondence to Rajendra Prasad Gujjeti.

Rights and permissions

This article is published under an open access license. Please check the 'Copyright Information' section for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team.

About this article

Verify currency and authenticity via CrossMark

Cite this article

Gujjeti, R.P., Namthabad, S. & Mamidala, E. HIV-1 reverse transcriptase inhibitory activity of Aerva lanata plant extracts. BMC Infect Dis 14, P12 (2014).

Download citation


  • Crude Extract
  • Reverse Transcriptase Activity
  • Density Gradient Centrifugation Method
  • Reverse Transcriptase Inhibitory Activity
  • Solvent Crude Extract