Volume 14 Supplement 3

Abstracts from the 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)

Open Access

HIV-1 reverse transcriptase inhibitory activity of Aerva lanata plant extracts

  • Rajendra Prasad Gujjeti1Email author,
  • Sainath Namthabad1 and
  • Estari Mamidala1
BMC Infectious Diseases201414(Suppl 3):P12

https://doi.org/10.1186/1471-2334-14-S3-P12

Published: 27 May 2014

Background

HIV-1 reverse transcriptase (HIV-1 RT) is an essential enzyme for the replication cycle of HIV. HIV-1 RT inhibitors have been extensively investigated for their anti-HIV properties. However, emergence of HIV drug resistance and side effects are the main reasons for failure of anti-HIV therapy. The aim of the present study was to evaluate the HIV reverse transcriptase inhibitory activity of Aerva lanata plant extracts.

Methods

Extracts were prepared from dried roots in different solvents. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy donors by ficoll-hypaque density gradient centrifugation method. A toxicity study was performed on all crude extracts among PBMCs by MTT assay. HIV-1 RT inhibition activity of the all solvent extracts of A. lanata was determined by a HIV-1 Reverse Transcriptase activity assay.

Results

All the five solvent crude extracts of A. lanata were non cytotoxic up to 0.75 mg/mL concentration in PBMCs. Chloroform and methanol extracts shows highest inhibition of recombinant HIV RT (91% and 89% respectively) at 1 mg/mL concentration. This strong inhibitory effect was confirmed by their IC50. More than 50% inhibition of HIV RT shows from 0.03 to 1 mg/mL concentrations of all extracts.

Conclusion

Experimental results thus suggested that the A. lanata plant extracts which have been tested in the present study exert their anti-HIV activity via inhibition of HIV Reverse Transcriptase activity. However, in order to assess the usefulness of this herb, it is necessary to isolate the active principle (s) from the crude extracts.

Authors’ Affiliations

(1)
Infectious Diseases Research Lab, Department of Zoology, Kakatiya University

Copyright

© Gujjeti et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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