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Volume 14 Supplement 3

Abstracts from the 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)

DC SIGNR silencing reduces HIV-1 infection in Dendritic cells

BMC Infectious Diseases volume 14, Article number: O8 (2014) Cite this article

Background

Dendritic cells (DCs) capture HIV-1 from periphery via DC SIGN/R receptors and transfer to T cells. It has been reported that siRNA directed against DC SIGN significantly inhibits HIV infection of DCs. In this study, the expression of DC SIGN/R and its role in HIV-1 infectivity of DCs were assessed.

Methods

DCs were cultured from monocytes from healthy donors and infected with HIV-1 Indian clade C virus. DC SIGN/R expression on DCs was determined by RealTime PCR. The effect of down regulation of this receptor in DCs using DC SIGN/R siRNA on the infection with HIV-1 was assessed. Statistical significance was calculated by Student’s t test.

Results

High expression of DC SIGN/R was observed on DCs infected with HIV-1 (p= 0.01). DC sIGNR expression in DCs was maximally downregulated by siRNA at 24 hrs (p=0.002) and was associated with significant reduction in expression of CD40 (p= 0.003), CD80 (p=0.008), CD86 (p=0.007) and P38 MAPK (p=0.005). Transfection of DC SIGNR on DCs at 24 hours followed by infection with clade C HIV-1 demonstrated lower levels of p24 compared to that in untransfected DCs (p=0.0008).

Conclusion

Data demonstrates that down regulation of DC SIGNR expression on DCs decreases activation that in turn may inhibit DC T cell interactions needed for progression of HIV-1 infection. Reduced p24 antigen production may be mediated by P38 MAPK inhibition. Our finding suggests that blocking DC SIGNR expression on DCs may prevent initial binding of HIV-1 and serve as a first step in prevention of HIV-1 infection.

Author information

Affiliations

  1. Dept. of Biochemistry, All India Institute of Medical Sciences, New Delhi, India

    Omkar Chaudhary, Sanjeev Kumar, Heena Aggarwal, Muzamil A Makhdoomi & Kalpana Luthra

  2. Dept. of Biochemistry, Kurukshetra University, Kurukshetra, India

    Jasbir Singh

  3. Blood Transfusion Services, Cardiothoracic and Neurosciences Center, All India Institute of Medical Sciences, New Delhi, India

    Jasbir Singh & Anjali Hazarika

Authors
  1. Omkar Chaudhary
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  2. Sanjeev Kumar
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  3. Heena Aggarwal
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  4. Muzamil A Makhdoomi
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  5. Jasbir Singh
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  6. Anjali Hazarika
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  7. Kalpana Luthra
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Corresponding author

Correspondence to Kalpana Luthra.

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Cite this article

Chaudhary, O., Kumar, S., Aggarwal, H. et al. DC SIGNR silencing reduces HIV-1 infection in Dendritic cells. BMC Infect Dis 14, O8 (2014). https://doi.org/10.1186/1471-2334-14-S3-O8

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Keywords

  • Public Health
  • Internal Medicine
  • Infectious Disease
  • Dendritic Cell
  • High Expression

BMC Infectious Diseases

ISSN: 1471-2334

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