Skip to main content

Investigation of anti-HIV activity, cytotoxicity and HIV integrase inhibitory activity of polyherbal formulation BH extracts


HIV integrase (IN) plays important roles at several steps, including viral DNA nuclear import, targeting viral DNA to host chromatin and integration. Identification of novel inhibitors of HIV Integrase has emerged as promising antiviral agents for the treatment of HIV/AIDS. Present work is to investigation of anti-HIV activity and HIV integrase inhibitory activity of various extracts of polyherbal formulation BH.


Polyherbal extracts (BH) were tested for anti-HIV activity against HIV-1 and -2 in MT-4 cells and cytotoxicity also tested against uninfected MT-4 cells. BH extracts were investigated for inhibition of HIV integrase enzymatic activity to understand the mechanism of antiviral action.


All extracts exhibited inhibitory activity against HIV-1 integrase (3’P IC50: 8.8-63 μg/mL and ST IC50: 4.9-65 μg/mL). The ethanolic extract (BH-HT) displayed significant inhibitory activity against both step of HIV in enzymatic activity (3’P IC50: 8.8µg/mL and ST IC50: 7.5 µg/mL). The ethanolic extract also inhibits the HIV-1 replication at the concentration of 59.30 µg/mL and cytotoxicity was found to be more than >125 µg/mL.


All the extracts inhibit the HIV integrase activity and ethanolic extract inhibit HIV and Integrase enzyme.

Author information

Authors and Affiliations


Corresponding author

Correspondence to S Balraj.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Balraj, S., Selvam, P., Pommier, Y. et al. Investigation of anti-HIV activity, cytotoxicity and HIV integrase inhibitory activity of polyherbal formulation BH extracts. BMC Infect Dis 14 (Suppl 3), O23 (2014).

Download citation

  • Published:

  • DOI:


  • Enzymatic Activity
  • Inhibitory Activity
  • Ethanolic Extract
  • Antiviral Action
  • Antiviral Agent