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BMC Infectious Diseases

Open Access

Investigation of anti-HIV activity, cytotoxicity and HIV integrase inhibitory activity of polyherbal formulation BH extracts

  • S Balraj1Email author,
  • P Selvam2,
  • Y Pommier3,
  • M Metifiot3,
  • M Christophe3,
  • C Pannecouque4 and
  • E De Clercq4
BMC Infectious Diseases201414(Suppl 3):O23

https://doi.org/10.1186/1471-2334-14-S3-O23

Published: 27 May 2014

Background

HIV integrase (IN) plays important roles at several steps, including viral DNA nuclear import, targeting viral DNA to host chromatin and integration. Identification of novel inhibitors of HIV Integrase has emerged as promising antiviral agents for the treatment of HIV/AIDS. Present work is to investigation of anti-HIV activity and HIV integrase inhibitory activity of various extracts of polyherbal formulation BH.

Method

Polyherbal extracts (BH) were tested for anti-HIV activity against HIV-1 and -2 in MT-4 cells and cytotoxicity also tested against uninfected MT-4 cells. BH extracts were investigated for inhibition of HIV integrase enzymatic activity to understand the mechanism of antiviral action.

Results

All extracts exhibited inhibitory activity against HIV-1 integrase (3’P IC50: 8.8-63 μg/mL and ST IC50: 4.9-65 μg/mL). The ethanolic extract (BH-HT) displayed significant inhibitory activity against both step of HIV in enzymatic activity (3’P IC50: 8.8µg/mL and ST IC50: 7.5 µg/mL). The ethanolic extract also inhibits the HIV-1 replication at the concentration of 59.30 µg/mL and cytotoxicity was found to be more than >125 µg/mL.

Conclusion

All the extracts inhibit the HIV integrase activity and ethanolic extract inhibit HIV and Integrase enzyme.

Authors’ Affiliations

(1)
Hans Rover Herbal Clinic, Perambalur, Tamil Nadu, India
(2)
Nova College of Pharm. Edu and Research, Jupudi, AP, India
(3)
Laboratory of Molecular Pharmacology, NCI, USA
(4)
Rega Institute for Medical Research, Leuven, Belgium

Copyright

© Balraj et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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