Volume 14 Supplement 3

Abstracts from the 2nd International Science Symposium on HIV and Infectious Diseases (HIV SCIENCE 2014)

Open Access

A novel gyrB gene mutation in fluoroquinolone resistant clinical isolates of Mycobacterium tuberculosis

  • Pooja Singh1,
  • Amita Jain1Email author,
  • Pratima Dixit1,
  • Shantanu Prakash1,
  • Indu Jaiswal1,
  • Vimala Venkatesh1 and
  • Mastan Singh1
BMC Infectious Diseases201414(Suppl 3):O14

https://doi.org/10.1186/1471-2334-14-S3-O14

Published: 27 May 2014

Introduction

Fluoroquinolone (FQ) resistance in Mycobacterium tuberculosis can be conferred by mutations in gyrA or gyrB gene. Recent studies suggest that amino acid substitutions in gyrB gene may also play a crucial role in resistance, but genetic studies of these mutations in M. tuberculosis are lacking.

Methods

A total of 100 ofloxacin resistant (OFX r) and 100 OFX sensitive (OFX s) isolates of M.tuberculosis isolates were consecutively selected from routine TB laboratory during 2012-2013. All the isolates were screened for phenotypic OFX r (>2µg/ml) by 1% proportion method and tested for minimal inhibitory concentration by absolute concentration method. Quinolone resistance determining region (QRDR) of gyrA and gyrB genes of 320bp and 428bp respectively were amplified, sequenced and compared with M.tuberculosis H37Rv.

Results

Mutations in the gyrB gene were observed in 5 of the 100 OFX r isolates. The single nucleotide mutation sites were in codons 538, 500, 539 (in two isolates) and 592. In one isolate, a substitution at codon 592 (Pro592Ser) was found as novel mutation outside QRDR region of gyrB gene. Accession nos. of these isolates include; KF509920-KF509922, KC880086 & KC880101. All the isolates showing mutations in gyrB gene also had mutations in gyrA gene. Mutations in gyrA gene were observed in 79% OFX r isolates. No mutation was observed in gyrB gene of OFX s isolates.

Conclusion

No OFX r isolates had shown mutation in the gyrB gene in the absence of gyrA gene mutation. The role of the gyrB gene mutation in conferring resistance to OFX in M.tuberculosis needs to be studied further.

Authors’ Affiliations

(1)
Department of Microbiology, King George’s Medical University

Copyright

© Singh et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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