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Virulence determinants of MRSA causing skin and soft tissue infections among HIV patients from Chennai
BMC Infectious Diseases volume 14, Article number: E44 (2014)
MRSA is a well armed pathogen with an array of virulence factors, which causes skin and soft tissue infections (SSTIs) among healthy individuals in community. Although there are few reports on MRSA causing SSTIs among HIV patients, there are no studies on their virulence determinants. Hence, the current study was done to detect the virulence determinants of MRSA causing SSTIs among HIV patients.
A total of 70 clinical isolates of Staphylococcus aureus collected from HIV patients with SSTIs were screened for MRSA using cefoxitin disc (30µg). Detection of pvl, femA, mecA of the isolates was done by triplex PCR. The enterotoxins A–E, J, K, P and egc cluster, exfoliatins A,B and D, hemolysins α, β, γ, δ & γv, leucocidins lukDE & lukM and innate immune evasions were detected by using multiplex PCRs using suitable S. aureus controls.
Of the 70, 16 (22.85%) of the S. aureus isolates causing SSTIs among HIV patients were found to be MRSA. pvl was detected in 15/16(93.75%) of MRSA isolates. 14/22(58.33%) virulence determinants tested were detected among the MRSA isolates. Among enterotoxins, sea and egc gene cluster were found in all MRSA isolates tested. 11/16(68.75%) and 10/16(62.50%) MRSA isolates were found to be positive for chp and lukD-E, respectively.
Although the MRSA percentage among HIV patients was low, prevalence of pvl and other virulence determinants was found to be high among MRSA isolates causing SSTIs in HIV patients.
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Krishnan, P., Nagarajan, A. & Suriakumar, J. Virulence determinants of MRSA causing skin and soft tissue infections among HIV patients from Chennai. BMC Infect Dis 14, E44 (2014). https://doi.org/10.1186/1471-2334-14-S3-E44
- Infectious Disease
- Healthy Individual
- Gene Cluster
- Staphylococcus Aureus
- Virulence Factor