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  • ePoster presentation
  • Open Access

Virulence determinants of MRSA causing skin and soft tissue infections among HIV patients from Chennai

BMC Infectious Diseases201414 (Suppl 3) :E44

  • Published:


  • Infectious Disease
  • Healthy Individual
  • Gene Cluster
  • Staphylococcus Aureus
  • Virulence Factor


MRSA is a well armed pathogen with an array of virulence factors, which causes skin and soft tissue infections (SSTIs) among healthy individuals in community. Although there are few reports on MRSA causing SSTIs among HIV patients, there are no studies on their virulence determinants. Hence, the current study was done to detect the virulence determinants of MRSA causing SSTIs among HIV patients.


A total of 70 clinical isolates of Staphylococcus aureus collected from HIV patients with SSTIs were screened for MRSA using cefoxitin disc (30µg). Detection of pvl, femA, mecA of the isolates was done by triplex PCR. The enterotoxins A–E, J, K, P and egc cluster, exfoliatins A,B and D, hemolysins α, β, γ, δ & γv, leucocidins lukDE & lukM and innate immune evasions were detected by using multiplex PCRs using suitable S. aureus controls.


Of the 70, 16 (22.85%) of the S. aureus isolates causing SSTIs among HIV patients were found to be MRSA. pvl was detected in 15/16(93.75%) of MRSA isolates. 14/22(58.33%) virulence determinants tested were detected among the MRSA isolates. Among enterotoxins, sea and egc gene cluster were found in all MRSA isolates tested. 11/16(68.75%) and 10/16(62.50%) MRSA isolates were found to be positive for chp and lukD-E, respectively.


Although the MRSA percentage among HIV patients was low, prevalence of pvl and other virulence determinants was found to be high among MRSA isolates causing SSTIs in HIV patients.

Authors’ Affiliations

Department of Microbiology, Dr. ALM PGIBMS, University of Madras, Chennai, India
Food Analysis Laboratory, TNFSDA, Madurai, India
Govt. Hospital of Thoracic Medicine, Tambaram, Chennai, India
Institute of Microbiology, Madurai Medical College, Madurai, India


© Krishnan et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.