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  • ePoster presentation
  • Open Access

HbAHP-25, a peptide designed in silico, exhibits potent anti-HIV activity in vitro

BMC Infectious Diseases201414 (Suppl 3) :E30

https://doi.org/10.1186/1471-2334-14-S3-E30

  • Published:

Keywords

  • Peptide
  • Tight Junction
  • Molecular Docking
  • Tight Junction Protein
  • Affect Cell Viability

Background

Identifying and / or designing molecules that can inhibit HIV infection and be safe to the host cells is highly desired.

Methods

HbAHP-25 was designed in silico against CD4 binding domain of gp120 of HIV-1 by molecular docking using Z dock and PROSA softwares. ELISA and SPR were used to determine the binding ability of HbAHP-25 to gp120. Anti-HIV activity of this peptide was checked by two different assays, viz: a) On TZM bl cells, using luciferase assay; b) On CEM-GFP cells and PBMCs using p24 antigen assay. MTT assay, TER/microsphere assay and Immunofluorescence were used to determine the effect of HbAHP-25 on cell viability, epithelial monolayer integrity and permeability.

Results

Five peptides were designed, and one of the peptides, HbAHP-25 , exhibits significant anti-HIV activity against various strains of HIV-1, such as HIV-1 Ada, HIV-1 NL4-3, and HIV-1 IIIB. ELISA and SPR revealed a direct interaction between HbAHP-25 and gp120, thereby inhibiting its interaction with CD4 receptor. The peptide didn’t affect cell viability even at higher concentrations; nor did it affect epithelial monolayer integrity or permeability. HbAHP-25 also did not interfere with any tight junction proteins such as ZO-1 and Clauddin-1, thus maintaining cell integrity as well.

Conclusion

The peptide has potent anti-HIV activity, and can be explored as a potential therapeutic /prophylactic/preventive agent.

Authors’ Affiliations

(1)
Molecular Immunology & Microbiology (MIM), National Institute for Research in Reproductive Health, Mumbai, India
(2)
Department of Bioinformatics, Dr. D.Y. Patil University CBD Belapur, Navi Mumbai, India

Copyright

© Bashir et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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