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  • ePoster presentation
  • Open Access

M. tuberculosis Peptide based Point-of care test for TB

  • Jesus M Gonzalez1, 2,
  • Bryan Francis1,
  • Sherri T Burda3,
  • R Sam Niedbala1, 2 and
  • Suman Laal1, 3, 4Email author
BMC Infectious Diseases201414(Suppl 3):E27

https://doi.org/10.1186/1471-2334-14-S3-E27

Published: 27 May 2014

Keywords

TuberculosisMycobacterium TuberculosisImmunodominant EpitopePeptide ConjugationPeripheral Health

Background

A rapid, simple and affordable point-of care (POC) test that can be implemented in peripheral health care settings to replace/improve upon microscopy for TB diagnosis or to rule out TB (triage) remains unmet.

Methods

Immunodominant regions of 3 Mycobacterium tuberculosis specific cell–wall proteins that are highly immunogenic in HIV- TB+ and HIV+TB+ patients have been mapped. Antibodies to these regions are absent in subjects with latent TB, BCG vaccination, other pulmonary diseases. To examine the feasibility of devising a peptide based rapid POC test, selected peptides were conjugated to a carrier protein using standard EDC/NHS coupling chemistry, purified by size exclusion; the peptide conjugation confirmed using MALDI MSMS before adsorption onto colloidal gold and striping onto nitrocellulose. The assay parameters were adjusted to determine positive/negative status within 15 minutes via visual or instrumented assessment.

Results

The prototype POC test was evaluated with sera from ~400 TB patients, non TB subjects from the groups defined in the methods section. The current prototype POC test provides >90% sensitivity and specificity in the above populations.

Conclusion

WHO recommends against use of all current commercial serological TB tests since they fail to achieve adequate sensitivity and specificity. These results demonstrate that, as for HIV rapid tests, carefully selected immunodominant epitopes of highly antigenic M. tuberculosis specific proteins can be the basis of antibody detection based rapid POC TB test. Test optimization to improve accuracy to meet WHO specifications for a POC test to replace microscopy or a triage test to rule-out TB are ongoing.

Authors’ Affiliations

(1)
TB Biosciences, Bethlehem, USA
(2)
Lehigh University, Department of Chemistry, Bethlehem, USA
(3)
Veterans Affairs Medical Center, New York Harbor Health Care System, New York, USA
(4)
New York University Langone Medical Center, Departments of Pathology and Microbiology, New York, USA

Copyright

© Gonzalez et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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