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  • ePoster presentation
  • Open Access

Mycobacterium bovis Bacille Calmette-Guerin infection modulates GRK2/3 dependent cytokine secretion

BMC Infectious Diseases201414 (Suppl 3) :E20

https://doi.org/10.1186/1471-2334-14-S3-E20

  • Published:

Keywords

  • Epithelial Cell
  • Tuberculosis
  • Western Blot Analysis
  • Initial Phase
  • Mycobacterium Tuberculosis

Background

Mycobacterium tuberculosis has evolved highly specialized mechanisms to proliferate in the host during infection. In this process, the infection of alveolar epithelial cells is a necessary step for mycobacteria dissemination; however the mechanisms of mycobacterial epithelial interactions are incompletely understood. Previously, we characterized the role of epithelial G protein coupled receptors (GPCR) CXCR1 and CXCR2 during mycobacterial infection. However the role of GPCR kinases (GRK) 2/3 and GRK4-6 in response to mycobacterial infection has not been investigated.

Methods

The GPCR kinases expression (GRK2/3 and GRK4-6) after Mycobacterium infection was quantified by RT-PCR and Western blot analysis. Further, the secretion of cytokines IL-8 and TNF-α was quantified in supernatants by ELISA.

Results

Mycobacterial infection in lung epithelial cells increased secretion of IL-8 and decreased TNF-α upto 72 hours. Further, the infection in the epithelial cells was modulated by a combined up regulation of GPCR kinases (GRK) 2/3 genes and suppression of the GRK 4-6 gene expression. These results were confirmed at protein levels. In addition, the blocking of chemokine receptors decreased the inhibition of GRK 2/3 expression suggesting that mycobacteria manipulate epithelial responses by desensitizing the receptors and the cytokine secretion.

Conclusions

In conclusion, we have identified a role for GRK 2/3 dependent cytokine secretion in the initial phase of mycobacterial infections in the lung epithelial cells.

Authors’ Affiliations

(1)
Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, Delhi, India
(2)
Department of Translational and Regenerative Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India

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