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  • Open Access

Ultra-deep sequencing of HIV-1 near full-length and partial proviral genomes among chronically infected blood donors at four blood centers in Brazil

  • 1,
  • 1,
  • 2,
  • 3,
  • 4,
  • 5,
  • 6 and
  • 1
BMC Infectious Diseases201414 (Suppl 2) :P61

https://doi.org/10.1186/1471-2334-14-S2-P61

  • Published:

Keywords

  • Blood Donor
  • Blood Center
  • Proviral Genome
  • Mina Gerais
  • Prevalent Subtype

Introduction

Here, we aimed to gain a comprehensive picture of HIV-1 diversity in the north-east and south-east part of Brazil. To this end, a high-throughput sequencing was used to characterize the near full length (NFLG) and partial HIV-1 proviral genome in blood donors at four major blood centers in Brazil: Pro-Sangue foundation (São Paulo state (SP), n 48), Hemominas foundation (Minas Gerais state (MG), n 41), Hemope foundation (Recife state (PE), n 97) and Hemorio blood bank (Rio de Janeiro (RJ), n 90).

Material and methods

From 2007-2011, 341 HIV+ blood donors from 4 blood centers were recruited to participate in a case control study to identify risk exposure. Of those, 294 specimens were classified as chronically infected subjects based on reactivity and positivity on the tests for recent HIV infection. Five overlapping amplicons spanning the HIV genome were PCR amplified from PBMCs. The amplicons were molecularly bar-coded, pooled, and sequenced by Illumina protocol.

Results

Of the 294 samples studied, 229 (77.9%) NFLGs and 50 (17%) partial fragments were de novo assembled into contiguous sequences and successfully subtyped. Of those, 200 (71.7%) were pure subtypes consisting of clade B (n = 151, 75.5%), C (n = 11, 5.5%) F1 (n = 4, 2%) and D (n = 3, 1.5%). Recombinant viruses were detected in 79 (28.3%) samples and consist of BF1 (n = 67, 84.8%), BC (n = 6, 7.6%), BCF1 (n = 4, 5%), CF1 and BUA1 (n = 1, 1.2%, each). Evidence of dual infection with the same subtype was detected in 1 patient. Two distinct BF1 recombinant profiles based on NFLG, with four samples in profile I and seven in profile II were detected and constitute two novel recombinant forms circulating in PE.

Conclusion

Subtype B appears to be the prevalent subtype in the north-east and south-east part of Brazil followed by a high proportion of intersubtype recombinants. These results provide insights into the understanding the genesis of HIV-1 epidemic in this particular area of South America and inform vaccine design and clinical trials.

Authors’ Affiliations

(1)
Institute of Tropical Medicine, University of São Paulo, São Paulo, Brazil
(2)
Pernambuco Hematology and Hemotherapy Institute - HEMOPE, Recife, PE, Brazil
(3)
Rio de Janeiro Hematology and Hemotherapy Institute - HEMORIO, Rio de Janeiro, Brazil
(4)
Federal University of Minas Gerais - UFMG, Belo Horizonte, MG, Brazil
(5)
Department of Infectious Diseases, University of São Paulo, São Paulo, Brazil
(6)
Blood Systems Research Institute, San Francisco, California 94117, USA

Copyright

© Pessôa et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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