Antimicrobial susceptibility/resistance and genetic characteristics of Neisseria gonorrhoeaeisolates from Poland, 2010-2012

Table 2 Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) STs, minimum inhibitory concentrations (MICs, mg/L) of cefixime and ceftriaxone, and penA alterations in Neisseria gonorrhoeae (n = 228) isolated in Poland, in 2010-2012

NG-MAST ST (No. of isolates)	Drug	No. of isolates with MIC (mg/L):					penA
		No. of isolates with MIC (mg/L):					alteration^a
		≤0.016	0.032	0.064	0.125	0.25
1407 (58)	IX		4	30	24		58 (100%) mosaic
1407 (58)	TX	4	18	27	9		58 (100%) mosaic
2992 (17)	IX	17					17 (100%) WT
2992 (17)	TX	17					17 (100%) WT
1405 (12)	IX	3	8	1			12 (100%) A501V
1405 (12)	TX	2	6	4			12 (100%) A501V
1861 (10)	IX	5	5				10 (100%) WT
1861 (10)	TX	1	4	5			10 (100%) WT
8392 (8)^b	IX	8					8 (100%) WT
8392 (8)^b	TX	8					8 (100%) WT
8379 (8)^b	IX	8					8 (100%) WT
8379 (8)^b	TX	6	2				8 (100%) WT
8391 (7)^b	IX	7					7 (100%) WT
8391 (7)^b	TX	7					7 (100%) WT
5421 (7)	IX	7					7 (100%) WT
5421 (7)	TX	7					7 (100%) WT
8370 (6)	IX	6					6 (100%) WT
8370 (6)	TX	6					6 (100%) WT
1478 (5)	IX	5					5 (100%) WT
1478 (5)	TX	5					5 (100%) WT
7674 (5)	IX	5					5 (100%) WT
7674 (5)	TX	5					5 (100%) WT
21 (5)	IX	4	1				5 (100%) WT
21 (5)	TX	2	1	2			5 (100%) WT
STs with 2-4 isolates (42)^c	IX	30	6	4	2		8 (19%) mosaic; 34 (81%) WT
STs with 2-4 isolates (42)^c	TX	31	6	5			8 (19%) mosaic; 34 (81%) WT
Unique STs (38)^d	IX	28	3	5	2		10 (26%) mosaic; 1 (2.6%) A501V; 1 (2.6%) A501T; 26 (68%) WT
Unique STs (38)^d	TX	28	4	4	2

NG-MAST, Neisseria gonorrhoeae multiantigen sequence typing; ST, sequence type; MIC, minimum inhibitory concentration; IX, cefixime; TX, ceftriaxone; WT, wild type.
^aPresence of penA mosaic allele or alteration of amino acid A501 in the penicillin-binding protein 2, which both have been associated with decreased susceptibility to extended-spectrum cephalosporins [5].
^bNovel sequence types found in the present study.
^cSTs represented by 2-4 isolates; 225 (4), 8387 (4)^b, 8373 (3)^b, 298 (3), 1340 (3), 8385 (3)^b, 4758 (3), 735 (2), 2226 (2), 5004 (2), 8377 (2)^b, 8378 (2)^b, 8393 (2)^b, 8394 (2)^b, and 8399 (2)^b.
^dUnique STs represented by single isolates.

ISSN: 1471-2334